Luisa Stefani and Rafael Matos have created tennis history to deny Sania Mirza a fairytale finish to her trailblazing career with victory in the Australian Open mixed doubles final.

The unseeded duo proved too strong for Mirza and fellow Indian Rohan Bopanna, prevailing 7-6 (7-2) 6-2 in Friday's championship decider at Rod Laver Arena.

In doing so, they became the first all-Brazilian pairing to win a grand slam title.

Mirza won six major doubles titles - three mixed, including the 2009 Australian Open - and will end her glittering career next month in Dubai.

The 36-year-old first appeared at a grand slam 18 years ago in Melbourne, when she was beaten by eventual champion Serena Williams in the women's singles third round.

"I've had the privilege to come back here again and again, and win some tournaments and play some great finals," Mirza said.

"Rod Laver Arena has really been special in my life and I couldn't think of a better arena to finish my career at in a grand slam."

Mirza was 14 years old when she first partnered Bopanna on court, and the close friends have since shared a special bond.

The 42-year-old Bopanna said Mirza had been an inspiration for tennis fans across the world, especially in her homeland.

"It's truly special for me to play along with Sania," Bopanna said.

"Unfortunately we couldn't get the title, but thank you so much for what you have done for Indian tennis."

Stefani and Matos dropped just one set - against the Australian wildcard pairing of Olivia Gadecki and Marc Polmans in their semi-final - across five matches that led to the championship.

It is the first time either 25-year-old Stefani or 27-year-old Matos have won a major title.

The pair came together earlier this year at the United Cup in Brisbane, and are now unbeaten in seven matches.

Stefani said an invitation from Matos to play together on her return from a serious knee injury provided inspiration as she completed her rehabilitation last year.

"It was about Wimbledon-time last year and he asked me how I was doing," she said.

"I was doing rehab ... that was a big motivation for me to keep working hard and keep getting closer to coming back.

"Now here we are, winning our first grand slam together in Australia in this beautiful court ... it's a dream come true."

Stefani and Matos vowed to be back next year to defend their crown.